基本素養 Basic Literacy
■ 生命科學之專業知識
The professional knowledges of life sciences
■ 科學精神及邏輯辯證
Scientific spirit and logic of dialectical
■ 培養同理心、尊重包容的公民素養
Develop the civic literacy of empathy, respect and inclusive
■ 對生命的社會關懷
The social care of lives
■ 生態保育的哲學觀與正義思維
The philosophy amd justice thinking of ecological conservation
■ 國際宏觀視野與掌握國際科研潮流
Global macro-vision and grasp the trend of international research
核心能力 Competence
■ 充分掌握生命科學專業知識的深度與廣度
Fully grasp the depth of the life sciences expertise
■ 慎密邏輯思考、計量分析與獨立決斷能力
Capable of thinking carefully and logically, measurement analysis and deciding independently
□ 學術交流與溝通能力
Academic communication and interpersonal skills
□ 跨領域知識整合與創新能力
Integration of cross-cutting knowledge and ability to innovate
■ 展現團隊合作及領導統御能力
Capacity to show teamwork amd leadership
課程概述 Course Description
本新課程將延續大學部課程“基因體理論與實作”之理念，加強碩士班學生基礎實驗訓練，並以探索式教學方式出發，培養學生獨立思考能力，並著重學生實際操作，發展系所特色，以建立南部生物科技中心。希望透過未來改進創新相關之生物技術課程，以整合性研究經驗帶動我國轉譯生物學人才的培育使命，並把有限的教育資源放在此一關鍵技術平台的傳授與推廣應用。課程學習目標 Course Objectives
課程進度 Progress Description
| 進度說明 Progress Description | |
|---|---|
| 1 | Introduction (何盧勳，張松彬，劉雅心，李純純)1. Micropipetting2. Bacterial culture techniques: preparation of LB broth & LB agar3. Sterilization |
| 2 | (1) 1.Mini-prep of plasmid DNA (1)2.Restriction analysis of DNA (1)3.DNA electrophoresis (1) 4.Isolation of DNA fragments 5.DNA ligation |
| 3 | 1.Cloning tools and strategies (2)2. Horizontal manipulation of genetic materials 1. Transformation 2. Preparation of agarose gel |
| 4 | Lecture: Properties of genetic materials: Chromatin and genomic level (張松彬，李純純)Practice: Isolation of genomic DNA, Fluorescence in situ hybridization |
| 5 | Practice: Restriction analysis of DNA, isolation and quantitation of DNA fragments, DNA ligation |
| 6 | Lecture: Horizontal manipulation of genetic materials (張松彬，李純純)Practice: Cell culture techniques, transformation, transfection, transduction |
| 7 | Group discuss and troubleshooting I (何盧勳，張松彬，劉雅心，李純純) |
| 8 | Practical bioinformatics (特別演講：李國彬副教授 國立陽明大學生物醫學資訊研究所) |
| 9 | Lecture: Reverse genetics (劉雅心)Practice: Isolation and quantitation of RNA, Reverse transcription |
| 10 | Lecture: Gene expression (RNA level) (劉雅心)Practice: Quantitative PCR, In vitro transcription |
| 11 | Group discuss and troubleshooting II (何盧勳，張松彬，劉雅心，李純純) |
| 12 | Proteomics (特別演講：游佳融副教授 私立長庚大學生物醫學研究所) |
| 13 | Lecture: Protein engineering (何盧勳)Practice: Protein extraction and fractionation, affinity chromatography, SDS-PAGE, Western blot assay |
| 14 | Lecture: Immunological bioassays in vitro (何盧勳)Practice: Immunoprecipitation assay |
| 15 | Lecture: Immunological bioassays in vivo (何盧勳)Practice: Immunohistological assay |
| 16 | Group discuss and troubleshooting III (何盧勳，張松彬，劉雅心，李純純) |
| 17 | Oral presentation and competition I (何盧勳，張松彬，劉雅心，李純純) |
| 18 | Oral presentation and competition II (何盧勳，張松彬，劉雅心，李純純) |
以上每週進度教師可依上課情況做適度調整。The schedule may be subject to change.
有關課程其他調查 Other Surveys of Courses
1.本課程是否規劃業界教師參與教學或演講?Is there any industry specialist invited in this course? How many times?
2.本課程是否規劃含校外實習(並非參訪)?
Are there any internships involved in the course? How many hours?