基本素養 Basic Literacy

生命科學之專業知識
The professional knowledges of life sciences
科學精神及邏輯辯證
Scientific spirit and logic of dialectical
培養同理心、尊重包容的公民素養
Develop the civic literacy of empathy, respect and inclusive
對生命的社會關懷
The social care of lives
生態保育的哲學觀與正義思維
The philosophy amd justice thinking of ecological conservation
國際宏觀視野與掌握國際科研潮流
Global macro-vision and grasp the trend of international research

核心能力 Competence

充分掌握生命科學專業知識的深度與廣度
Fully grasp the depth of the life sciences expertise
慎密邏輯思考、計量分析與獨立決斷能力
Capable of thinking carefully and logically, measurement analysis and deciding independently
學術交流與溝通能力
Academic communication and interpersonal skills
跨領域知識整合與創新能力
Integration of cross-cutting knowledge and ability to innovate
展現團隊合作及領導統御能力
Capacity to show teamwork amd leadership

課程概述 Course Description

微生物實驗課為本系大學部三年級之必修課程中,親手結合微生物學中之理論以及實質操作的實作型課程。 自98學年度起,本系即開始推動一系列的課程更新與調整,所有的實驗課程,已不同於以往是由助教帶領,而是改由本系專任教師親自帶領所有課程的進行;同時,在每學期實驗課程開始之前一個月,即必須開始訓練研究生小助教,以及提前測試新學期中所需之所有菌種、試劑、生化生理反應、所需儀器與器材的檢驗與測試,期望在開學之初,所有參與實驗課程的小助教已經具備有一定程度的基本學養與實驗進行與領導學生進行課程的能力。

課程學習目標 Course Objectives

  • .熟悉整個學期各週每一個微生物實驗的內涵、安全性、與實驗步驟
  • 各週實驗課開始前之前置作業,規劃提前作菌種活化、藥品、培養基的配置與確認的工作
  • 有領導各組學生正確執行實驗、疑難解答能力的培養與確保實驗可完整、安全進行的能力
  • 課程進度 Progress Description

    進度說明 Progress Description
    1Check in (確認事項:分組、打掃時間表與工作職掌、實驗衣確認、顯微鏡確認與初級測試、菌種表說明)
    2微生物實驗概論微生物相關基本實驗技術操作、儀器使用解說與規範、器材正確使用與清潔以及安全說明。
    3Basic laboratory techniques for isolation, cultivation, and cultural characterization of microorganisms
    4Exp.3 Cultural characteristics of microorganisms Maintenance and preservation of pure cultures
    5Exp.5 Microscopic examination of living microorganismsExp.6 The microscopic measurement of microorganismsExp.8 Preparation of bacterial smears
    6Exp.9 Simple stainExp.11 Gram stainExp.12 Acid-Fast stainExp.13 Spore stain
    7IntroductionExp.14 Nutritional requirements: media for the routine cultivation of bacteriaExp.15 Use of differential, selective, and enriched media
    8Exp.18 Physical factors: atmospheric oxygen requirementsExp.19 Techniques for the cultivation of anaerobic microorganisms
    9Exp.20 Serial dilution-agar plate procedure to quantitative viable cellExp.21 The bacterial growth curve
    10學期中討論與檢討
    11Biochemical activity of microorganisms: IntroductionExp. 22 Extracellular enzymatic activity of microorganismExp. 23 Carbohydrate fermentation
    12Exp. 25 IMViCExp. 26 Hydrogen sulfite testExp. 27 Urease testExp. 29 Nitrate reduction testExp. 32 Utilization of amino acid
    13Microbiology of water: Introduction Exp. 50 Standard qualitative analysis of waterExp. 51 Quantitative analysis of water: membrane filter method
    14ImmunologyExp. 72 Immuno-Fluorescence stain I
    15ImmunologyExp. 72 Immuno-Fluorescence stain II
    16Q-PCR 在微生物學界的應用與操作 I
    17Q-PCR 在微生物學界的應用與操作 II
    18期末檢討
     以上每週進度教師可依上課情況做適度調整。The schedule may be subject to change.

    有關課程其他調查 Other Surveys of Courses

    1.本課程是否規劃業界教師參與教學或演講? 否
    Is there any industry specialist invited in this course? How many times? No
    2.本課程是否規劃含校外實習(並非參訪)? 否
    Are there any internships involved in the course? How many hours? No