基本素養 Basic Literacy
■ 生命科學之專業知識
The professional knowledges of life sciences
■ 科學精神及邏輯辯證
Scientific spirit and logic of dialectical
■ 培養同理心、尊重包容的公民素養
Develop the civic literacy of empathy, respect and inclusive
■ 對生命的社會關懷
The social care of lives
■ 生態保育的哲學觀與正義思維
The philosophy amd justice thinking of ecological conservation
■ 國際宏觀視野與掌握國際科研潮流
Global macro-vision and grasp the trend of international research
核心能力 Competence
■ 充分掌握生命科學專業知識的深度與廣度
Fully grasp the depth of the life sciences expertise
■ 慎密邏輯思考、計量分析與獨立決斷能力
Capable of thinking carefully and logically, measurement analysis and deciding independently
□ 學術交流與溝通能力
Academic communication and interpersonal skills
□ 跨領域知識整合與創新能力
Integration of cross-cutting knowledge and ability to innovate
■ 展現團隊合作及領導統御能力
Capacity to show teamwork amd leadership
課程概述 Course Description
本新課程將延續大學部課程“基因體理論與實作”之理念，加強碩士班學生基礎實驗訓練，並以探索式教學方式出發，培養學生獨立思考能力，並著重學生實際操作，發展系所特色，以建立南部生物科技中心。希望透過未來改進創新相關之生物技術課程，以整合性研究經驗帶動我國轉譯生物學人才的培育使命，並把有限的教育資源放在此一關鍵技術平台的傳授與推廣應用。課程學習目標 Course Objectives
課程進度 Progress Description
| 進度說明 Progress Description | |
|---|---|
| 1 | Introduction (何盧勳，張松彬，劉雅心，李純純)1. Micropipetting 2. Bacterial culture techniques: preparation of LB broth & LB agar 3. Sterilization |
| 2 | (1) 1.Mini-prep of plasmid DNA (1)2.Restriction analysis of DNA (1)3.DNA electrophoresis (1) 4.Isolation of DNA fragments 5.DNA ligation |
| 3 | 1.Cloning tools and strategies (2) 2. Horizontal manipulation of genetic materials 1. Transformation 2. Preparation of agarose gel |
| 4 | Lecture: Properties of genetic materials: Chromatin and genomic level (張松彬，李純純)Practice: Isolation of genomic DNA, Fluorescence in situ hybridization |
| 5 | Practice: Restriction analysis of DNA, isolation and quantitation of DNA fragments, DNA ligation |
| 6 | Lecture: Horizontal manipulation of genetic materials (張松彬，李純純)Practice: Cell culture techniques, transformation, transfection, transduction |
| 7 | Group discuss and troubleshooting I (何盧勳，張松彬，劉雅心，李純純) |
| 8 | Practical bioinformatics (特別演講：李國彬副教授 國立陽明大學生物醫學資訊研究所) |
| 9 | Lecture: Reverse genetics (劉雅心)Practice: Isolation and quantitation of RNA, Reverse transcription |
| 10 | Lecture: Gene expression (RNA level) (劉雅心)Practice: Quantitative PCR, In vitro transcription |
| 11 | Group discuss and troubleshooting II (何盧勳，張松彬，劉雅心，李純純) |
| 12 | Proteomics (特別演講：游佳融副教授 私立長庚大學生物醫學研究所) |
| 13 | Lecture: Protein engineering (何盧勳)Practice: Protein extraction and fractionation, affinity chromatography, SDS-PAGE, Western blot assay |
| 14 | Lecture: Immunological bioassays in vitro (何盧勳)Practice: Immunoprecipitation assay |
| 15 | Lecture: Immunological bioassays in vivo (何盧勳)Practice: Immunohistological assay |
| 16 | Group discuss and troubleshooting III (何盧勳，張松彬，劉雅心，李純純) |
| 17 | Oral presentation and competition I (何盧勳，張松彬，劉雅心，李純純) |
| 18 | Oral presentation and competition II (何盧勳，張松彬，劉雅心，李純純) |
以上每週進度教師可依上課情況做適度調整。The schedule may be subject to change.
有關課程其他調查 Other Surveys of Courses
1.本課程是否規劃業界教師參與教學或演講? 否Is there any industry specialist invited in this course? How many times? No
2.本課程是否規劃含校外實習(並非參訪)? 否
Are there any internships involved in the course? How many hours? No