基本素養 Basic Literacy
■ 生命科學之專業知識
The professional knowledges of life sciences
■ 科學精神及邏輯辯證
Scientific spirit and logic of dialectical
□ 培養同理心、尊重包容的公民素養
Develop the civic literacy of empathy, respect and inclusive
□ 對生命的社會關懷
The social care of lives
□ 生態保育的哲學觀與正義思維
The philosophy amd justice thinking of ecological conservation
□ 國際宏觀視野與掌握國際科研潮流
Global macro-vision and grasp the trend of international research
核心能力 Competence
■ 充分掌握生命科學專業知識的深度與廣度
Fully grasp the depth of the life sciences expertise
■ 慎密邏輯思考、計量分析與獨立決斷能力
Capable of thinking carefully and logically, measurement analysis and deciding independently
□ 學術交流與溝通能力
Academic communication and interpersonal skills
□ 跨領域知識整合與創新能力
Integration of cross-cutting knowledge and ability to innovate
■ 展現團隊合作及領導統御能力
Capacity to show teamwork amd leadership
課程概述 Course Description
微生物實驗課為本系大學部三年級之必修課程中，親手結合微生物學中之理論以及實質操作的實作型課程。 自98學年度起，本系即開始推動一系列的課程更新與調整，所有的實驗課程，已不同於以往是由助教帶領，而是改由本系專任教師親自帶領所有課程的進行；同時，在每學期實驗課程開始之前一個月，即必須開始訓練研究生小助教，以及提前測試新學期中所需之所有菌種、試劑、生化生理反應、所需儀器與器材的檢驗與測試，期望在開學之初，所有參與實驗課程的小助教已經具備有一定程度的基本學養與實驗進行與領導學生進行課程的能力。課程學習目標 Course Objectives
在學生小助教參與完整的微生物學實驗教學課程後，可以培養出:1.熟悉整個學期各週每一個微生物實驗的內涵、安全性、與實驗步驟；
2.熟悉學期各週實驗課開始前之前置作業，規劃提前作菌種活化、藥品、培養基的配置與確認的工作。
3.在技術本質學能熟練之外，帶領小助教有領導各組學生正確執行實驗、疑難解答能力的培養與確保實驗可完整、安全進行的能力。
課程進度 Progress Description
| 進度說明 Progress Description | |
|---|---|
| 1 | Check in (確認事項:分組、打掃時間表與工作職掌、實驗衣確認、顯微鏡確認與初級測試、菌種表說明) |
| 2 | 微生物實驗概論 微生物相關基本實驗技術操作、儀器使用解說與規範、器材正確使用與清潔以及安全說明。 |
| 3 | Basic laboratory techniques for isolation, cultivation, and cultural characterization of microorganisms: Introduction Exp.1 Culture transfer techniques Exp.2 Techniques for isolation of pure cultures Maintenance and preservation of pure cultures |
| 4 | Exp.3 Cultural characteristics of microorganisms Maintenance and preservation of pure cultures |
| 5 | Exp.5 Microscopic examination of living microorganisms Using a hanging-drop preparation or a wet mount Exp.6 The microscopic measurement of microorganisms Exp.8 Preparation of bacterial smears |
| 6 | Bacterial stain: Introduction Exp.9 Simple stain Exp.11 Gram stain Exp.12 Acid-Fast stain Exp.13 Spore stain |
| 7 | Cultivation of microorganisms: Introduction Exp.14 Nutritional requirements: media for the routine cultivation of bacteria Exp.15 Use of differential, selective, and enriched media |
| 8 | Exp.18 Physical factors: atmospheric oxygen requirements Exp.19 Techniques for the cultivation of anaerobic microorganisms |
| 9 | Exp.20 Serial dilution-agar plate procedure to quantitative viable cell Exp.21 The bacterial growth curve |
| 10 | 學期中討論與檢討 |
| 11 | Biochemical activity of microorganisms: Introduction Exp. 22 Extracellular enzymatic activity of microorganism Exp. 23 Carbohydrate fermentation |
| 12 | Biochemical activity of microorganisms Exp. 25 IMViC Exp. 26 Hydrogen sulfite test Exp. 27 Urease test Exp. 29 Nitrate reduction test Exp. 32 Utilization of amino acid |
| 13 | Microbiology of water: Introduction Exp. 50 Standard qualitative analysis of water Exp. 51 Quantitative analysis of water: membrane filter method |
| 14 | Immunology Exp. 72 Immuno-Fluorescence stain I |
| 15 | Immunology Exp. 72 Immuno-Fluorescence stain II |
| 16 | Q-PCR 在微生物學界的應用與操作 I |
| 17 | Q-PCR 在微生物學界的應用與操作 II |
| 18 | 期末檢討 |
以上每週進度教師可依上課情況做適度調整。The schedule may be subject to change.
課程學習融入下列議題或具有下列內涵的程度
Immersing the Following Issues or Contents
| 議題或內容 Issues or Contents | 關聯性 Correlation |
|---|---|
| 性別平等 Gender Equity | 無相關 No correlation |
| 法治教育 Law-Related Education | 無相關 No correlation |
| 人權教育 Human Rights Education | 無相關 No correlation |
| 服務學習 Service Learning | 直接相關 Direct correlation |
| 生命教育 Life Education | 無相關 No correlation |
| 智慧財產權 Intellectual Property | 無相關 No correlation |
| 環境安全 Environmental Safety | 無相關 No correlation |
| 環境保護 Environmental Protection | 無相關 No correlation |
| 文創產業 Cultural and creative Industry | 無相關 No correlation |
| 健康醫療照護產業 Health,Medical Treatment, Nursing Industry | 間接相關 Indirect correlation |
| 精緻農業產業 Advanced Agriculture | 無相關 No correlation |
| 生物科技產業 Biotechnology Industry | 間接相關 Indirect correlation |
| 觀光旅遊產業 Tourism | 無相關 No correlation |
| 綠色能源產業 Green Energy Industry | 無相關 No correlation |
| 強化工業基礎技術方案 Advancing basic industry technology program | 無相關 No correlation |
| 學術研究取向 Academic Orientation | 無相關 No correlation |
| 工作實務取向 Pragmatic Orientation | 直接相關 Direct correlation |
有關課程其他調查 Other Surveys of Courses
1.本課程是否規劃業界教師參與教學或演講? 否Is there any industry specialist invited in this course? How many times? No
2.本課程是否規劃含校外實習(並非參訪)? 否
Are there any internships involved in the course? How many hours? No